Julianne A.
Private tutor in Philadelphia, PA
Education
-University of Pennsylvania, Philadelphia, PA – Post Baccalaureate Program, August 2014 – June 2016 GPA: 3.75 ∙ Coursework: Cognitive Neuroscience, Neurobiology of Behavior, Physiology of Motivated Behavior, Animal Minds -Widener University, Chester, PA - BS in Biochemistry, Honors Certification, May 2013 GPA: 3.92
Experience
Teaching Experience: - University of Pennsylvania: Aug 2014 - Present •Teaching undergraduate, graduate and post-doctoral trainees lab techniques •Techniques taught: colony maintenance and husbandry, breeding of novel genetic lines, immunofluorescence, light microscopy, injection techniques, fear conditioning, T-maze training, PCR, cardiac perfusions and tissue harvest - Widener Works Externship Program: Jan 2016 and Jan 2017 •Hosted biochemistry student currently enrolled at Widener University and allowed them to shadow my work for a day -Temple University: Sept 2013 – Nov 2013 •Organic Chemistry I Lab Teaching Assistant - Widener University: Sept 2010 - May 2013 •General Chemistry and Psychology 101 Tutor Research Experience: University of Pennsylvania under Dr. Steve Thomas: March 2014 -Present - Mouse Colony General Maintenance and Genotyping: •Organization of multiple genetic lines, husbandry, PCR •Breeding of novel genetic lines with specific requirements for reproduction and survival - Effect of Corticosterone on Working Memory in Mice: •T-Maze positive reinforcement with pharmacological manipulations - Quantification of DNA in Novel Genetic Lines: •Brain tissue harvest, RNA extraction, cDNA generation, qPCR - Quantification of Corticosterone Levels During Restraint Stress in Mice: •Blood collection, corticosterone assay (ELISA) - Activation of Reporter Genes Using Tamoxifen: •Subcutaneous and Intraperitoneal injection techniques •Cardiac perfusion, cryostat specimen preparation and slicing/staining of brain tissue •General tissue harvest (brain, spleen, adrenal glands) •Quantification through: Immunofluorescence, light microscopy, western blotting - Contextual and Cued Fear Conditioning in Mice: •Intraperitoneal injection technique, sterile technique, handling and habituating mice •Use of classical conditioning apparatus and recording results based on observations •Cannulation survival surgery and infusion of drugs directly into dorsal hippocampus Temple University under Dr. William Wuest: May-Aug 2013 -Natural Products •Bacterial flow systems, plating, bacterial inhibition testing, MIC determination •Bacterial Inhibition Compounds patent pending and publication Widener University under Dr. Krishna Bhat: 2012-2013 -Spectroscopic Analysis of Commercial Resveratrol Samples: June-Aug 2012 •Developed and defended original thesis; techniques included IR and NMR •Presented ideas, methods, and findings to faculty and students •Won First Place Summer Research Poster Award 2012 - Density Calculations and Chemical Analysis of Resveratrol (Senior Thesis): June 2012 – May 2013 •Distillation, solvent prep, Density Meter 5000, density and sound velocity calculations
Availability
Weekends at any time
Can Meet
Up to 25 minutes away at my regular hourly rate
Hobbies
I am currently leaving my position at the University of Pennsylvania due to moral issues with animal research. I would like to pursue the subject of animal behavior and eventually go back for my doctorate in the subject. I love animals, music, and crafting.
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